TOPICS

2020/06/08
phenomics-based drug discovery

2020/05/26
Zebrafish-Based Precision Oncology

2020/05/25
Zebrafish-Based Phenotype Screening

2020/05/23
phenomicsはzebrafishにより爆発する。

2020/02/21
High-Throughput Cardiotoxicity Screening System for Onco-Cardiology

》Microinjection into Zebrafish Embryos

                     
2011/06/09

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

関連ファイル

Embryo injection performed by L. Zang.