TOPICS

2022/09/15
患者がん移植ゼブラフィッシュモデルとプレシジョンメディシン

2022/07/13
がんの個別化医療を、目指して

2022/07/02
ハイスループット心毒性スクリーニングを、開始しました。

2022/06/02
個別化医療への道程

2021/10/26
ゼブラフィッシュ創薬からがんの個別化医療を実現へ

》Microinjection into Zebrafish Embryos

                     
2011/06/09

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

関連ファイル

Embryo injection performed by L. Zang.