TOPICS

2023/03/14
ゼブラフィッシュ創薬の国際的展開

2023/03/02
17社のゼブラフィッシュ創薬支援CROのグローバル展開

2022/09/30
患者がん移植ゼブラフィッシュモデル(PDXZ)と プレシジョンメディシン

2022/09/15
患者がん移植ゼブラフィッシュモデルとプレシジョンメディシン

2022/07/13
がんの個別化医療を、目指して

》Microinjection into Zebrafish Embryos

                     
2011/06/09

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

関連ファイル

Embryo injection performed by L. Zang.