TOPICS
2018/09/11
ゼブラフィッシュ創薬とプレシジョンメディシン
2018/08/31
ゼブラフィッシュ創薬とプレシジョン医療
2018/08/16
爆発する世界のゼブラフィッシュ論文
2018/08/05
ゼブラフィッシュ創薬とブレシジヨン メディシン
2018/07/01
ゼブラフィッシュ創薬とプレシジョンメディシン
》Microinjection into Zebrafish Embryos
2011/06/09
Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.
Commented by Y. Shimada