TOPICS

2025/12/11
PDXZは、創薬スクリーニングを革新する。

2025/12/10
2026年1月21日(水)AIプレシジョンPDXZzoomセミナー

2025/12/09
PDXZの放射線増感作試験 (Radio-sensitization)

2025/12/08
PDXZによる固形がん(Solid Tumor)増殖モデルの構築

2025/12/04
薬効解析のためのPDXZ移植部位PVS (Perivitelline Space: 卵黄周囲腔)

》Microinjection into Zebrafish Embryos

                     
2011/06/09

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

関連ファイル

Embryo injection performed by L. Zang.