TOPICS
2025/06/23
患者がん移植ゼブラフィッシュモデル(PDXZ)の臨床応用
2025/06/11
ゼブラフィッシュ創薬の急激なグローバル発展
2025/05/02
新規蛍光色素ZMB741によるポドサイトパチー治療薬スクリーニング
2025/04/15
ポドサイトパチー創薬スクリーニング
2025/04/10
なぜ患者がん移植ゼブラフィッシュモデル(PDXZ)は、有効か
》Microinjection into Zebrafish Embryos
2011/06/09
Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.
Commented by Y. Shimada