TOPICS

2024/04/25
新しい心毒性防御遺伝子であるstanniocalcin1

2024/04/24
PDXZの腫瘍血管新生解析

2024/04/23
ゼブラフィッシュ骨加齢モデルによる創薬研究プロジェクト

2024/04/22
Zebrafish Yolk Sac Microinjection of Medicine

2024/04/04
21世紀ゼブラフィッシュ創薬発展の必要条件

》Microinjection into Zebrafish Embryos

                     
2011/06/09

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

関連ファイル

Embryo injection performed by L. Zang.