2020/06/08
phenomics-based drug discovery
2020/05/26
Zebrafish-Based Precision Oncology
2020/05/25
Zebrafish-Based Phenotype Screening
2020/05/23
phenomicsはzebrafishにより爆発する。
2020/02/21
High-Throughput Cardiotoxicity Screening System for Onco-Cardiology
Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.
Commented by Y. Shimada