TOPICS
2023/03/14
ゼブラフィッシュ創薬の国際的展開
2023/03/02
１７社のゼブラフィッシュ創薬支援CROのグローバル展開
2022/09/30
患者がん移植ゼブラフィッシュモデル(PDXZ)と プレシジョンメディシン
2022/09/15
患者がん移植ゼブラフィッシュモデルとプレシジョンメディシン
2022/07/13
がんの個別化医療を、目指して
》Microinjection into Zebrafish Embryos
2011/06/09
Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.
Commented by Y. Shimada