TOPICS
2025/10/16
AIDXプレシジョン患者がん移植システムによるゼブラフィッシュ創薬と個別化医療
2025/10/07
AIプレシジョン患者がん移植ゼブラフィッシュシステムの急展開
2025/10/02
臨床治療応答予測のためのin vitro診断ツール：PDXZ
2025/06/23
患者がん移植ゼブラフィッシュモデル(PDXZ)の臨床応用
2025/06/11
ゼブラフィッシュ創薬の急激なグローバル発展
》Microinjection into Zebrafish Embryos
2011/06/09
Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.
Commented by Y. Shimada