Publication List English

Generation of a Transgenic Zebrafish Line for In Vivo Assessment of Hepatic Apoptosis

Patient-Derived Cancer Xenograft Zebrafish Model (PDXZ) for Drug Discovery Screening and Personalized Medicine

Quality Control Protocol for Zebrafish Developmental Toxicity Studies

Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish

A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis

tEffects of Yuzu (Citrus junos Siebold ex Tanaka) peel on the diet-induced obesity in a zebrafish model


Liqing Zang, Yasuhito Shimada, Jumpei Kawajiria, Toshio Tanaka, Norihiro Nishimura

Received 7 March 2014, Revised 31 July 2014, Accepted 7 August 2014, Available online 6 September 2014

Journal of Functional Foods (2014),
pp. 499-510 DOI information: 10.1016/j.jff.2014.08.002

Effects of yuzu peel (Citrus junos Siebold ex Tanaka), yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with diet-induced obesity (DIO) were evaluated. All materials tested exhibited anti-obesity effects. Yuzu peel significantly suppressed the rise in plasma triacylglycerol (TG) and liver lipid accumulation. The hepatic mRNA expression of pparab (peroxisome proliferator-activated receptor, alpha b) and its target genes were significantly upregulated by yuzu peel, which suggests enhanced fatty acid ƒΐ-oxidation in liver. In visceral adipose tissue, yuzu peel significantly increased the mRNA expression of pparg (peroxisome proliferator-activated receptor, gamma) and adipoqb (adiponectin, C1Q and collagen domain containing, b), which play roles in adipose differentiation and maintenance. Our findings suggest that yuzu peel exerts anti-obesity effects by activating hepatic PPARƒΏ and adipocyte PPARƒΑ pathways. Additionally, the anti-obesity effects of yuzu pomace suggest a novel application to achieve complete use of yuzu instead of disposal as industrial waste.


Journal title:Journal of Functional Foods


Article title: Effects of Yuzu (Citrus junos Siebold ex Tanaka) peel on diet-induced obesity in a zebrafish model