Publication List English

2021/10/31
Generation of a Transgenic Zebrafish Line for In Vivo Assessment of Hepatic Apoptosis

2021/08/19
Patient-Derived Cancer Xenograft Zebrafish Model (PDXZ) for Drug Discovery Screening and Personalized Medicine

2021/07/09
Quality Control Protocol for Zebrafish Developmental Toxicity Studies

2020/10/13
Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish

2020/05/28
A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis

tPatient-Derived Cancer Xenograft Zebrafish Model (PDXZ) for Drug Discovery Screening and Personalized Medicine

                     
2021/08/19

The U.S. National Cancer Institute has been using bank-derived cell lines for drug screening for many years, but due to the lack of clinical efficacy of this 2D cell culture drug screening (<5%), it was discontinued in 2016, and patient-derived xenograft mouse model (PDXM, >80% clinical drug efficacy predictive capacity) has been rapidly developed internationally.

However, the PDXM requires severe immunodeficient mice, and it takes about one year to obtain quantitative drug efficacy test results, and many issues remain, such as cost.

We developed novel zebrafish model for patient-derived xenograft (PDXZ), and demonstrated that PDXZ has high rate and speed of engraftment in normal zebrafish when the immune system is immature, requires less than 100 patient cancer cells for transplantation, and can be quantitatively analyzed in 2 days. We report on the construction of a drug efficacy screening system that can be quantitatively analyzed within a week.

ŠΦ˜AƒŠƒ“ƒN

BioJapan2021

Department of Systems Pharmacology, Mie University Graduate School of Medicine

ŠΦ˜Aƒtƒ@ƒCƒ‹

Patient-Derived Cancer Xenograft Zebrafish Model (PDXZ) for Drug Discovery Screening and Personalized Medicine