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2021/07/09
Quality Control Protocol for Zebrafish Developmental Toxicity Studies

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Gap junction protein beta 4 plays an important role in cardiac function in humans, rodents, and zebrafish

2020/05/28
A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis

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C3orf70 Is Involved in Neural and Neurobehavioral Development

2019/09/22
Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation

tMolecular Cloning of Novel Mouse and Human Putative Citrate Lyase.

                     
2001/01/01

J.Morikawa,Y.Nishimura,A.Uchida, T.Tanaka
Biochem. Biophys. Res. Commun. 289:1282-1286 2001

Abstract

Using a fluorescent differential display (FDD) technique, a novel cDNA was identified by screening for gene expressed differentially between the Dunn osteosarcoma cell line and the LM8 cell line, an isolated variant of the Dunn cell line that has high metastatic potential to the lung. Molecular cloning of the cDNA revealed the clone has similarity to a bacterial fermentation enzyme, the citrate lyase beta-subunit (CL-beta). Northern blot and competitive reverse transcription-PCR (RT-PCR) analysis revealed up-regulation of the gene in the LM8 cell line. An RNA Master blot indicated that the mRNA encoding CL-beta is expressed abundantly in murine heart, liver, and kidney. A human expressed sequence tag (EST) database search suggested that a similar cDNA is expressed in humans. A gene with identical sequence is located on chromosome 13 in the genome database (Sanger centre, UK). These data suggest that a citrate fermentation pathway may exist in eukaryotes including mammals.

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Pubmed