Publication List English

A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis

C3orf70 Is Involved in Neural and Neurobehavioral Development

Generation of a Triple-Transgenic Zebrafish Line for Assessment of Developmental Neurotoxicity during Neuronal Differentiation

Aging-associated microstructural deterioration of vertebra in zebrafish

Zebrafish yolk sac microinjection of thalidomide for assessment of developmental toxicology

tGenomic organization,chromosomal localization,and alternative splicing of the human phosphodiesterase 8B gene.


M.Hayashi,Y.Shimada,Y.Nishimura,T.Hama, T.Tanaka
Biochem. Biophys. Res.Commun. 297:1253-1258 2002


We have characterized the gene for human phosphodiesterase 8B, PDE8B, and cloned the full-length cDNA for human PDE8B (PDE8B1) and two splice variants (PDE8B2 and PDE8B3). The PDE8B gene is mapped to the long arm of chromosome 5 (5q13) and is composed of 22 exons spanning over approximately 200kb. The donor and acceptor splice site sequences match the consensus sequences for the exon-intron boundaries of most eukaryotic genes. PDE8B1 encodes an 885 amino acid enzyme, containing an N-terminal REC domain, a PAS domain, and a C-terminal catalytic domain. PDE8B2 and PDE8B3 both have deletion in the PAS domain and encode 838 and 788 amino acid proteins, respectively. RT-PCR analysis revealed that while PDE8B1 is the most abundant variant in thyroid gland, PDE8B3, but not PDE8B1, is the most abundant form in brain. These findings suggest that selective usage of exons produces three different PDE8B variants that exhibit a tissue-specific expression pattern.