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2024/06/20

The world's most powerful high-throughput individualized in vivo proteinuria screening system

The world's most powerful high-throughput individualized in vivo proteinuria screening system is reported below.

In Vivo Assessment of Individual and Total Proteinuria in Zebrafish Larvae Using the Solvatochromic Compound ZMB741 | Chemical & Biomedical Imaging https://pubs.acs.org/doi/10.1021/cbmi.4c00029

2013 (ACS Chem Neurosci. 4:1183-93), we independently developed a novel fluorescent dye ZMB741 that rapidly binds to plasma proteins of all molecular weights in vivo when added to zebrafish rearing water and demonstrated its overwhelming superiority in the non-invasive, individual in We have developed a non-invasive, non-invasive zebrafish individual in vivo proteinuria quantification protocol for nephrotoxic proteinuria. As a result, we report the creation of an in vivo high-throughput nephrotoxicity screening system based on a completely new principle that can discriminate between glomerular and urinary repopulation disorders of nephrotoxicity.

We expect that this system will be utilized in a wide range of fields in the future.
(1) High-throughput screening for nephrotoxicity and analysis of nephrotoxicity mechanism
(1) Aristolochic Acid, (2) Monacolin K, Citrinin
(3) Puromycin Aminonucleoside (PAN)
(2) High-throughput screening of prophylactic regulators for nephrotoxicants and
　　(1) Resveratrol
(1)Resveratrol
(3) Pathogenetic analysis of human proteinuria disease model
(1)Glomerular Basement Membrane (GBM) Genes: laminin, collagen IV
(2)Podocyte-specific Genes: nephrin(nphs1), podocin(nphs2)
(4) High-throughput screening and mechanism of action analysis of therapeutic agents for human proteinuric diseases

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