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2011/12/19

A Novel Protocol for the Oral Administration of Test Chemicals to Adult Zebrafish　(管理者)

To cite this article:
Liqing Zang, Daizo Morikane, Yasuhito Shimada, Toshio Tanaka, and Norihiro Nishimura. Zebrafish. December 2011, 8(4): 203-210. doi:10.1089/zeb.2011.0726.
Published in Volume: 8 Issue 4: December 19, 2011

Zebrafish. 2011 Dec;8(4):203-10.
A novel protocol for the oral administration of test chemicals to adult zebrafish.
Zang L, Morikane D, Shimada Y, Tanaka T, Nishimura N.
Liqing Zang,1
Daizo Morikane,1
Yasuhito Shimada,2,3,4
Toshio Tanaka,2,3,4 and
Norihiro Nishimura1
1Department of Translational Medical Science, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
2Department of Molecular and Cellular Pharmacology, Pharmacogenomics, and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
3Department of Bioinformatics, Mie University Life Science Research Center, Tsu, Mie, Japan.
4Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Tsu, Mie, Japan.

Abstract
Abstract A novel protocol using gluten as a carrier material was developed to administer chemicals to adult zebrafish, per os (p.o.). To evaluate the capacity of gluten to retain chemicals, we prepared gluten granules containing eight types of chemicals with different Log P(ow) values and immersed them in water. Less than 5% of chemicals were eluted from gluten granules within 5 min, a standard feeding time for zebrafish. Although retention capability was dependent on the hydrophilicity and hydrophobicity of the chemicals, the gluten granules retained 62%-99% of the total amount of chemical, even after immersion in water for 60 min. Vital staining dyes, such as 4-Di-2-Asp and Nile red, administered p.o., were delivered into the gastrointestinal tract where they were digested and secreted. Subsequently, we conducted a pharmacokinetic study of oral administration of felbinac and confirmed that it was successfully delivered into the blood of zebrafish. This indicates that chemicals administered using gluten granules are satisfactorily absorbed from the digestive tract and delivered into the metabolic system. The absorption, distribution, and pharmacokinetics of chemicals given by oral administration were also compared with those of chemicals given by alternative administration routes such as intraperitoneal injection and exposure to chemical solution.
PMID: 22181663 [PubMed - in process]