TOPICS

2018/06/07
Zebrafish-Based Drug Discovery and Systems Pharmacology

2017/12/01
ƒtƒGƒmƒ~ƒNƒXŒย•ส‰ปˆใ—ร‚๐ŽภŒป

2017/11/25
Global Development in emerging zebrafish-based galternativeh methods

2017/11/25
ICH and 3Rs with zebrafish-based alternative methos

2017/11/23
171123“๚–{“ฎ•จŽภŒฑ‘ใ‘ึ–@Šw‰๏‘ๆ 30 ‰๑‘ๅ‰๏ƒVƒ“ƒ|ƒWƒEƒ€‚U

tMicroinjection into Zebrafish Embryos

                     
2011/06/09

Microinjection remains the most popular and effective of the methods to introduce antisense Morpholino, plasmid, RNA, proteins and chemicals into fertilized zebrafish eggs. A microinjection grass needle is filled with the Morpholino solution (with marker dye, such s phenol-red) and attached to an apparatus (e.g. FemtoJet) that forces the solution out of the needle with air pressure. A small amount of solution in then expelled into the cytoplasm of the embryo before withdrawing the pipet, and the injected embryos are incubated to develop further.

Commented by Y. Shimada

Šึ˜Aƒtƒ@ƒCƒ‹

Embryo injection performed by L. Zang.