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2021/07/09
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2020/05/28
A novel orexin antagonist from a natural plant was discovered using zebrafish behavioural analysis
Junya Kuroyanagia, Yasuhito Shimadaa, Beibei Zhanga, Michiko Ariyoshia, Noriko Umemotoa, Yuhei Nishimurua, Toshio Tanaka
Received 4 May 2014, Revised 29 June 2014, Accepted 28 July 2014, Available online 10 August 2014
DOI: 10.1016/j.febslet.2014.07.033
Abstract
Zinc finger, MYND-type containing 8 (ZMYND8) encodes a receptor for activated C-kinase protein. Here, we report that ZMYND8 promotes angiogenesis in prostate cancer xenografts in zebrafish, as well as tube formation in human umbilical vascular endothelial cell (HUVEC) cultures. Using transcriptome analyses, we found upregulation of ZMYND8 expression in both zebrafish prostate cancer xenografts and prostate cancer samples from patients. In vitro and in vivo ZMYND8 knockdown suppressed angiogenesis, whereas ZMYND8 overexpression enhanced angiogenesis. Notably, ZMYND8 induced vegfa mRNA expression selectively in prostate cancer xenografts. Integrated analysis of human and zebrafish transcriptomes, which identified ZMYND8, might be a powerful strategy to determine also other molecular targets for inhibiting prostate cancer progression.